Serial Analysis of Gene Expression (SAGE) SAGE is a powerful tool that allows the analysis of overall gene expression patterns with digital analysis.  Because SAGE does not require a preexisting clone, it can be used to identify and quantitate new gene as well as know genes.   cDNA/AFLP (CWB Bachem)The protocol which follows gives a step-by-step description of cDNA-AFLP, a method for visualisation of differential gene expression·         What\'s Differential Display (GenHunter)Introduction to differential display technique·         Differential Display (Chun-Ming Liu)The following procedures are described:o        RNA extraction and qualificationo        DNase treatment of RNA sampleo        Reverse transcriptiono        PCR amplificationo        Separation on acrylamide gelso        DNA extraction from bands of interesto        Re-amplification by PCRo        Separation on agarose gelso        Excision of amplified products  ·         Differential Display (Breeden Lab)Detailed protocol for differential display·         Differential Display (Plant Molecular Biolgy Lab)It\'s for plant RNA display. The protocol should be general.·         Differential Display (PDF) (Clontech)Provides detailed guide to differential display technique·         Differential Display-Reverse Transcription-PCR (Gerard Lazo)Optimized Welsh DDRT-PCR Protocol (Gerard Lazo)      Direct Characterization, Enrichment and Sequencing of Differential Displayed cDNA Sequences (TTO) Present a rapid and cost-effective approach without recourse to cloning for direct characterization of differential cDNA molecules, up to the point of sequence identification. The experimental conditions have been optimized so that the protocol could be reliably performed with only three steps of minimal manipulation.  ·         A simple method for screening cDNAs arising from the cloning of RNA differential display bands (TTO) This simple method allows to separate the obtained clones in subpopulations according to their sequence, thus reducing considerably the number of required reconfirmatory northern blot or reverse northern dot-blot reactions.·         Rapid, nonradioactive differential display using Tth polymerase (TTO) A rapid method to produce RNA fingerprints that can be also used for differential display·         Differential mRNA display using anchored oligo-dT and long sequence-specific primers as arbitrary primers (TTO)   抗原抗体反应的应用领域 2021-05-20 09:39网络 基因分离克隆的方法 2020-09-08 12:22互联网 RT-PCR在基因表达（gene expression）检测中的应用 2020-09-07 13:50互联网 DNA重组及基因工程技术对医学和生命科学发展的贡献 二 2020-08-24 22:56互联网 哺乳动物细胞基因稳定转染的实验 2020-08-10 20:51互联网 四环素作为可诱导基因表达的调控物实验 2019-09-12 10:05互联网 反向遗传学技术及其在FMDV 研究中的应用 2019-08-09 15:47蚂蚁淘 PCR技术应用二： 骨肿瘤诊断 2019-05-20 19:23蚂蚁淘 cDNA宏阵列方法分离拟南芥的臭氧应答基因实验(一) 2019-03-26 17:05互联网 哺乳动物细胞基因稳定转染的实验 2019-03-25 21:55互联网